KAYE, Thomas, Prospect Heights, IL; SAWLOWICZ, Zbigniew, Jagiellonian Univ.,
Krakow, Poland; GAUGLER, Gary, Microtechnics Inc., Granite Bay, CA; STIPANOVIC,
Bozidar, APL Biopurification Technologies, Highland Park, IL
Prompted by recent excitement in the scientific community surrounding the discovery
of soft tissues in Tyrannosaurus rex bone, a survey was undertaken in an attempt
to discover these tissues in situ. A variety Lance and Hell Creek specimens
including triceratops, hadrosaur, ankylosaur, champtosaur and well-preserved
small bones were examined under the scanning electron microscope (SEM) and energy
dispersive spectroscopy (EDS). Many likely structures were identified and found
in surprisingly common numbers. Micron-sized
spheres containing iron and oxygen were identified as an oxidized sub-group
of common sulfur-iron framboids. Filamentous mats and non-bone coatings were
imaged in the open vascular canals showing evidence of microbial movement though
a viscous film. Characteristic micron size pores on both the canal walls and
the framboid surfaces, suggested the presence of desiccated biofilms. Quartz
crystals with attached framboids suggested
a prolonged time line for framboid and biofilm development. Further analysis
after digestion in acid, showed that these structures resembled presumed dinosaurian
soft tissues but this study suggested that they were in fact microbiologic and
mineral in origin with a morphology that could allow for multiple interpretations.
Additional surveys of ammonoids from the Pierre Shale and bone from the White
River Group also contained these same structures indicating that morphology
alone is not a reliable identification method for soft
tissues. Consequently, this survey suggests, that a biologic testing regiment
is required to determine affinities and ages of biomollecules.